September 15, 1978 
Proposed Revisions to the NIH Guidelines for Research on Recombinant DNA 
Gentlemen: 
I am a research scientist in the Biology Department of the Brookhaven 
National Laboratory. In 1970 I received a degree (Ph.D.) in Microbiology 
from Washington University, St. Louis. For the past eight years my research 
has been concerned with molecular aspects of the replication of two DNA 
tumor viruses, human adenovirus (type 2) and simian virus 40 (SV40), and 
hybrids between these two viruses. Adenovirus-SV40 hybrids have been clas- 
sified by the NCI as moderate-risk tumor viruses, and studies utilizing 
these viruses require procedures and containment facilities similar to those 
specified in the present NIH recombinant DNA guidelines for P3 containment. 
My laboratory is a P3 containment laboratory and has been operated as such 
for the past three years. Although my actual experience with recombinant 
DNA has not been extensive, I have been involved with three projects over 
the past four years that have involved the cloning of specific DNA fragments 
or the use of recombinant DNAs. At present, however, I am not involved in 
any research involving recombinant DNA. About a year ago I organized a 
symposium held at the Brookhaven National Laboratory which considered the 
prospects for the introduction, retention, replication, and expresssion of 
foreign DNAs in eucaryotic cells. Many of the ideas, techniques, and 
results which were discussed at that symposium involved recombinant DNA 
technology. 
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