Dr. Donald S. Fredrickson 
August 17, 1978 
Page Two 
It is to be noted that the requirement to post the universal 
biohazard sign on freezers and refrigerators has been omitted 
from paragraph I I-B-4-a-(l 5) , although paragraph 1 1 -B-4-a-(8 ) 
does provide for the removal of viable or intact biological 
materials from a P4 facility. It is suggested the requirement 
for identifying storage units be included in paragraph 
II-B-4-a-(15). 
3. It is suggested that the reference to Horsfall units in 
paragraph II-B-3-b-(2) be cited in the bibliography. 
The appropriate citation is: Horsfall, F. L., Jr. and J. H. 
Baner (1940) Individual Isolation Of Infected Animals In 
A Single Room. J. Bact. 40, 569-580. 
4. In paragraph II-B-3-c-(7)(line 8), it is suggested that 
the word recirculation be replaced with the word reconditioning . 
This suggestion is made since it is apparently the intent 
of this provision to permit the installation of conditioning 
units in rooms having large heat loads in order to reduce 
the ventilation requirements . Thus, the word reconditioning 
would reflect the intent to permit treatment of the air 
relative to temperature control rather than the movement 
of air as is indicated by the word recirculation . 
It is also suggested that paragraph 3, under P3 Level 
(pp. 3314), Proposed Action; Environmental Impact Assessment 
be amended to reflect the reconditioning of air. 
5. It is suggested that the word sterilized be used in lieu of 
the word decontaminated in paraaraphs I I-B-l -a - (3 ) , 
I I-B-2-a-(3) , 1 1 -B-3-a - ( 3 ) , and II-B-4-a-(3) . 
The suggestion to use the word sterilized is based on the 
precision offered in relation to the destruction of viable 
orqanism in contrast to decontamination which refers to the 
removal of a contaminant. The word sterilized also serves 
to identify processes or methods for effecting the destruction 
of viable organisms. 
The foregoing are offered as constructive comment and in the 
interest of contributing to the conduct of research with re- 
combinant DNA molecules. You and your staff are to be complimented 
for havina developed a comprehensive and rational set of guidelines. 
Sincerely, 
Robert W. McKinney, Ph.D. 
RWMcK: kgr 
[A-23] 
