AREA CODE 6 0 0 
PHONE 262-2177 
McARDLE LABORATORY 
FOR CANCER RESEARCH 
UNIVERSITY OF WISCONSIN • MADISON, WISCONSIN 53706 
August 31 , 1978 
Dr. Donald S. Fredrickson 
Dl rector 
National Institutes of Health 
Bethesda , HD 2001 A 
Dear Don: 
Thank you for your letter of July 2k. I have read the proposed guidelines 
for recombinant DNA research especially those portions dealing with animal viruses 
and cells, an area In which I have expertise. 
I regard the guidelines dealing with the cloning of animal viruses and 
eukaryotic genomes Into E_. col 1 Kl 2 as reasonable. I do not believe that any 
harm can come from experiments carried out as described In the guidelines. In 
fact, I wonder about the necessity of regulating these experiments at all beyond 
the regulations required for work with the viruses and eukaryotic genomes directly. 
As our knowledge of vertebrate genomes and viruses Increases, we are 
finding fundamental differences between them and coll . These differences 
make It even less likely than was previously believed that the viral or vertebrate 
genes can be expressed In E. col I unless special manipulations have been performed. 
However, In respect to the use of animal viruses as cloning vectors, I still 
have some hesitation about the use of so many animal viruses that can replicate 
In and transform human cells. Clearly there Is no hazard to work with these 
viruses In cell cultures. However, I see no reason now not to restrict this cloning 
work to rodent and bird viruses and cells. 
Two minor points: "Genomes" Is mlspelled "genomes". In the impact statement: 
I do not think It Is fair to call cloned hormones genes when they are from cDNA 
from a vertebrate messenger mRNA. The clone lacks all before and after and 
Intervening sequences. 
I hope that the guidelines will be promulgated In the present form or In 
an even less restrictive form. 
With best regards, 
Sincerely yours, 
Howard M. Temln 
HMT : kjd 
[A-67] 
