THE UNIVERSITY OF ARIZONA 
ARIZONA MEDICAL CENTER 
TUCSON, ARIZONA 85724 
COLLEGE OF MEDICINE 
DEPARTMENT OF MICROBIOLOGY 
(602) 626-6061 
September 20, 1978 
Dr. Donald Fredrickson 
Director 
National Institutes of Health 
Bethesda, Maryland 20016 
Dear Dr. Fredrickson: 
I have read the proposed Revised Guidelines for Recombinant DNA Research 
and would like to express my approval of them. I must say that when 
the original guidelines came out I was skeptical of claims by many that 
recombinant DNA research posed no hazard. In light of more recent findings 
concerning the feebleness of IE. coli K12 and particularly regarding the 
structure of eukaryotic genes, my earlier misgivings have largely been 
allayed. While I believe that some precautions are still necessary, 
these do appear to be amply embodied in the proposed revision, and I 
fully agree with the general relaxing of standards. My own work with 
recombinant DNA involves coli genes cloned into E. coli , a system which 
worries nobody to my knowledge; I believe that removing this and similar 
systems from being covered by the Guidelines is a realistic step and 
will do much to foster credibility within the scientific community. 
Sincerely yours. 
A 
v 
John Little, Ph.D. 
Adjunct Assistant Professor 
JL:kb 
[A-217] 
