4 
Comments on the NIH guidelines -- References 
1. R. Curtiss III, Biological Containment and Cloning Vector 
Transmissibility . J. Inf. Pis . 137 , 668 (1978) 
2. S. B. Levy, Emergence of Antibiotic Resistant Bacteria in the 
Intestinal Fiora of Farm Inhabitants. J. Inf. Pis. 137 , 688 (1978) 
3. Letter to Dr. Emil Mrak, EPA, from Martin Alexander, Ad Hoc 
Study Group on recombinant DNA policy, Science Advisory Board, 
April 28, 1978 
4. E.S. Anderson, Plasmid Transfer in E. coli. J. Inf. Dis. 137, 
686 (1978) 
5. A speculation — footnote #13 in Freder ickson ' s introduction-- 
is that major structural features found in all genes of 
higher organisms and not bacteria will prevent the full 
expression of eukaryotic genes in an E. coli host. The 
universality of these structural features can hardly be 
be inferred from the limited data now available. Furthermore, 
it is easy to see how biologically active material could in any 
case be produced from portions of these cloned eukaryotic 
genes . 
6. J. King, Recombinant DNA and Auto-Immune Disease. J. Inf. Dis. 
137, 663, 1978 
7. Walker and Isselbacher, Gastroenterology 67 , 531 (1974) 
8. I. Jakobsson & T. Lindberg, Cow's Milk as a Cause of Infantile 
Colic in Breast-Fed Infants. The Lancet 26 August 1978, p. 437 
9. S.B. Levy, N. Sullivan, and S. L. Gorbach, Pathogenicity of 
conventional and debilitated E. coli K12. Nature 274 , 395 (1978) 
[A-373] 
