Appendix VIII 
i:\cerpted from The Recombinant-DNA Debate by Dr. Clifford Grobstein. 
Copyright ®19 y 7 by Scientific American, Inc . All rights reserved. 
BKXOGtCAL CONTAINMENT (FOR E COU MOST SYSTEMS ONLY) 
e*t 
E*2 
EX 3 
DNA kom nonpadiogonc proAaryotaa rtm 
nokjrwt, t i t»«ng» ganaa «• E CO t 
P-asnaa o t Mcmcpi^o. Dna kom neat 
cat rial nae^aP y aacnan pa o anat amr 
f cot it ptotmo or tacmc t /w 
3 #nor-a :ow*ni ’■*■■*> a rm o> t 
DNA m q ihw « xr 9$ n»c*m 
»/« and cnaracten.iad N p fiar a. an at 
CQntamman/ era ra qunafl | 
ONA fcoffl oNr cotd-Qioodad mniM end 
DNA kom noomtfyon t ooto-tsoodad 
ONA from modarata-naa ptncgtrac 
nanmd n na laoor M ory tor Im> 
n to grarMorai 
y t E cot 
DNA kom plants laacapt ptants contanrg 
tixat' paVtogana v proOLrcmg tnom 
xrxamgax proaaryotaa rial 
ONA from ttsa na * paPioqais c proaaryotaa 
rat oatatai aicnanga ganaa MX £ oof 
Qra a nata Dna kom non p maia 
auaar v otaa * Fpr or q araaa Bna nai a 
aaa nan W p a»cant p ixa N g nat a»as ot 
DNA kom 
do not tat /d| 
E cot 
ONA from plant nrusas 
Oa antM Dna ton prmatas I For 
jro anaN N> i w N" W 
p/t l ya> am o< corumrt 
ara 'aqua ad i 
Waantd or oacKnoona oa ONA kpm neat 
cat rat do not latxiN t^an at 
a rnaa a*f cot i » »a>t imN 
- ncratw ptregtsty 
or OCOO OKO p otano# pt np» i tagrjr 
avaN of comanmant art 'aounad ) 
DNA trow nonpafioganc proaaryotaa 
•at do not Mu d ) a a taga ganaa 
aa> E cot 
QNA kgm p ant rn^aa 
Ptasnaa or oacarpp nao a ONA kom non 
<* » —» y* 7* *^tro t Ural 
■*1 to* 1 * *«t« a j nia rat 
■a nc.ntt Mraotntty or 
ONA kom amdryon c pnmaa IIH .1 or 
DNA kom norartr«nc pnmaa I 
tgg!« ? No na am 
<* cpntannant at 'aNrrad I 
ONA kom oaar mammakan caaa 
ONA kom badi 
ONA kom amOryora c n onanOry o n c or 
•• 0* ’ 
"I 
ONA kpm anmai rruaaa | t oorad ONA 
garaaT 
ONA kom noflak a naa patiogarac 
pronaryotat rat do not naaxaay aicnanga 
ganaa •» E cot 
ONA kom aramat nrutas i « gonad DNA 
doaa not conar Harmful aanaa . 
ONA kom nonamoryorac pnmaaa kaaua 
’ DNA kpm anmai .ruaaa < ctpnad DNA 
SHOTGUN EXPERIMENTS USING E COU X-12 OR ITS DERfV 
ATIVES AS THE MOST CELL ANO PLASMlOS BAC1 
OR other viruses as the Cloning vectors 
Mi H EXPERIMENTS IN WHICH PURE CHARACTERIZED FOREIGN 
ATIVES AS THE HOST CELL ANO PLASMOS BACTERIOPHAGES GENES CaRRiE 0 By PlaSM'OS SACTERiOPhaGES OR'^ThER 
VIRUSES ARE Cloned IN £ CPU < '? OR ITS DERIVATIVES 
I Hr Hi PJ, n a mi At tmayoat ra p a r - 
ItlMI IXAMflXJ at ik« pkyatcal a ad H.lagtraf caatataaaat ra- 
i aal fartk tm Ms MH pMfci fa raarrk a.at->ta« ra- 
l ON A aataaata. I aaad la laaa. 1*7*. at pira a tfca ta- 
aMidaa. afcak rrpMcad tk« 
at tka yarattal rkto 
ONA n y « a nk C: 
•attia. A 
DNA 
a. kaawa a rickaagr ( .. a wtek F. call la l 
at.faada at |aad lat.ralary prartH. ipty 
nitHkaaMatka itaatirt C-1J ktiniiry aaaa at £. rati Ikta- 
m«irai raatataaaat Hr it mu Bataaaa Na. mrtaa Na MH 
fntrUan praarrNa M P t pM i n attai i a at la rr aaaa phyak 
.1 .ad t u ilipral raataaaaat fa tacnaatag Ira at aadmatat rlak. 
»• »*• i- ItcTMMi frwi l«#V !• b«« rtekt.) 
k» H £12 rWart U tW m W ‘ert f 
ihMi |n«(k ItfMli iMlfd to 
- a 1 . A 1.1 II ■ at a Ha 
■ ■•WlWtJ ■MIIM; 
ad fa aa UUnd kaat-rarta iyv 
t addklaaal MM 
Na .ary kaMad a. II > Mary at A* fardMa aad 
kaak.acaa qd ia kaa yat kaaa rarttMd ky Na NTH a atkdytag 
Na U) crunk Na racn I DNA iiparf i ak aaa la praf 
raa la Na t'A aaa E. eati Ha ly a taa ara aN a fra asrafidaaa 
leatady aaty Na patyaaa aad IVad kail) 
ra^atra Mtkar Na H m Na N led at pkyatcal cMklaaaM. Es- 
yanant «ak plaal ika kaal tyalaaa ka». apatial pA y d r al n a 
Na fl 
VIII-1 
[ 495 ] 
