low, we will nonetheless assay for such an occurrence by measuring for reverse 
transcriptase In the medium of transduced TIL prior to infiision into the patient. 
D . Limitation of the Usefulness of Aminoglycoside Antibiotics . 
The NeoR gene product, neomycin phosphotransferase (NPT) , 
phosphorylates the 3 '-hydroxyl group of the aminohexose I of neomycin and its 
analogues, thereby inactivating the antibiotic. While amikacin may be 
inactivated by this enzyme, gentamicin and tobramycin do not contain an hydroxyl 
at the 3' position and are not inactivated (24,25). Therefore, introduction of 
the NeoR gene would not exclude the use of aminoglycosides or any other 
conventional antibiotic that may be needed in the clinical management of these 
patients . 
IX. STATISTICAL CONSIDERATIONS 
Not applicable. 
X. REFERENCES ^ 
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J.A., Eglitis , M..A. , McLachlin, J.R., Wiginton, D.A., Hutton, J.J. Horowitz, 
S.D., Gilboa, E. , Blaese, R.M. , and Anderson, W.F. (1986). Correction of 
adenosine deaminase deficiency in cultured human T and B cells by 
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6) Kantoff, P.W. , Gillio, A.P. , McLachlin, J.R., Bordignon, C. , Eglitis ,, M.A. , 
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