1.5.1 In Vivo Experiments with the Murine T-helper cell clone 14.1 Transduced with 
the hADA Gene 
These data were provided during the extensive review of the N2/TIL protocol approved January 
19, 1989. In summary, these murine T cells were readily transduced with the SAX vector to 
express both neoR and hADA. Following ip injection of 25x10® cells into nude mice, T cells 
expressing the hADA gene were found in direct spleen lysates and NeoR expressing cells could be 
recovered by culture and selection in G418 for up to 83 days after transplantation. The 14.1 
cells are specific for the antigen SWM and the nude mouse recipients of these cells were able to 
produce antibody to SWM demonstrating the preservation of their intrinsic immune function 
when the cells were reinjected in vivo. Thus there was partial correction of immune function 
in these immunodeficient mice following transfer of \L2 expanded lymphocytes. See Appendix 
13.3, Figure 1. 
1 .5.2 In Vivo Experiments with Rhesus T-lymphocytes Transduced with the N2 and 
SAX Vectors 
T lymphocyte lines were established from adult Rhesus monkeys by stimulation of blood or 
lymph node cells with polyclonal activators followed by culture in rlL2 supplemented medium. 
Genes were introduced into these cells by retroviral-mediated gene transfer and the cells 
selected for expression of the NeoR gene in G418. Following infusion of from 5.4 xIO^ to 1.4 x 
1 0® transduced T cells/kg, NeoR gene-expressing cells could be recovered from these animals 
as late as 158 days after transfer. A summary of these ongoing monkey studies is shown in 
Appendix 13.3, Figure 2. 
1.5.3 In Vive Experiments in Immuncdeficient BNX Mice Injected with ADA(-)SCID 
Lymphccytes Transduced with the hADA Gene 
Our collaborator Claudio Bordignon (see attached letter) has studied the function of ADA 
(-)SCID lymphocytes injected into immunodeficient mice carrying the bg/nu/xid (BNX) 
mutation. Freshly isolated ADA(-)PBL were stimulated with antigen or PHA and transduced 
with the hADA gene by multiple cycles of exposure to ADA vector supernatant. 20x10® cells 
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