g. Pregnancy test if woman between the ages of 16 and 50 
h. Chest x-ray 
i. Electrocardiogram 
j. Baseline x-rays and nuclear medicine scans to evaluate the 
status of disease, 
k. CT scan or MRI scan of brain 
l. 45 ml of clotted blood for serum storage and 45 ml of anti- 
coagulated blood for mononuclear cell cryopreservation. Selected patients may 
undergo pretreatment lymphocytapheresis. 
m. Biopsy of tumor, if possible with minimal morbidity 
2. During treatment . Patients will have a complete blood count and 
chemistry analysis panel at least every other day and a chest x-ray performed 
each week during treatment. 
During the infusion of the transduced cells, patients will be monitored 
closely in the Surgical Intensive Care Unit, Vital signs including blood 
pressure, pulse, and respirations will be measured every 15 minutes during the 
cell infusion and every 30 minutes for at least four hours or until the patient 
is stable. A pulse oximeter will be used for on-line measurement of oxygen 
saturation during and for the four hours after cell infusion as well. If the 
systolic blood pressure drops below 80 mm/Hg, or the oxygen saturation drops 
below 90% during the cell infusion, the cell infusion will be terminated 
immediately, 
3. Post-treatment (see Table, page 17). Complete evaluation of evaluabl 
lesions with physical examination, biopsy, if feasible, and appropriate x-rays 
and/or scans prior to each cell infusion cycle and at approximately eight weeks 
after the end of treatment to evaluate response to treatment. 
Western blot analysis of patient serum to determine possible exposure to 
retrovirus envelope proteins will be performed at 7 to 8 weeks after treatment. 
Recombinant DNA Research, Volume 14 
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