Table 4 
Studies of Human TIL Transduced with TNF-NeoR Vector 
Patient Patient Patient 
NV 
1 (B) 
TNF/NEO 
NV 
2 (S) 
TNF/NEO 
3 (P) 
TNF/NEO 
Southern blot 
for TNF 
- 
+ 
- 
+ 
- 
+ 
TNF secretion* 
(pg/10^ cells/24hrs) 
35 
720 
17 
231 
16 
390 
Growth in G418 
No 
Yes 
No 
Yes 
No 
Pending 
Growth after IL-2 
withdrawal 
No 
No 
No 
No 
No 
No 
*TNF secretion assessed on day 25, day 35, day 33 after transduction on cells from 
tients 1, 2 and 3 respectively. 
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