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Appendix C that have built-in environmental limitations that permit optimum 
growth in the industrial setting but limited survival without adverse consequences 
in the environment. For those organisms that do not qualify for GILSP, the BLl- 
LS (Biosafety Level 1--Large-Scale) level of physical containment is reconunended 
for large-scale research or production of viable organisms containing recombinant 
DNA molecules that require BLl containment at the laboratory scale. The BL2- 
LS (Biosafety Level 2-Large Scale) level of physical containment is required for 
large-scale research or production of viable organisms containing recombinant 
DNA molecules that require BL2 containment at the laboratory scale. The BL3- 
LS (Biosafety Level 3-Large Scale) level of physical containment is required for 
large-scale research or production of viable organisms containing recombinant 
DNA molecules that require BL3 containment at the laboratory scale. No 
provisions are made for large-scale research or production of viable organisms 
containing recombinant DNA molecules that require BL4 containment at the 
laboratory scale. If necessary, these requirements will be established by NIH on 
an individual basis. 
•Appendix K-II-GILSP Level. 
"Appendix K-II-A. Cultures of viable organisms containing recombinant DNA 
molecules shall be handled in facilities intended to safeguard health during work 
with microorganisms that do not require containment. Processes and equipment 
should be designed and constructed to assure the integrity of the production 
organism and resulting product. 
"Appendix K-II-B. Addition of materials to a system, sample collection, transfer 
of culture fluids within/between systems, and processing of culture fluids shall be 
conducted in a manner that maintains employee exposure to viable organisms 
containing recombinant DNA molecules at a level that does not adversely affect 
the health and safety of employees. 
"Appendix K-II-C. Written instructions and training of personnel shall be 
provided to assure that cultures of viable organisms containing recombinant DNA 
molecules are handled prudently and that the workplace is kept clean and orderly. 
"Appendix K-II-D. In the interest of good personal hygiene, facilities (e.g., 
handwashing sink, shower, changing room) and protective clothing (e.g., uniforms, 
laboratory coats) shall be provided that are appropriate for the risk of exposure 
to viable organisms containing recombinant DNA molecules. In addition, eating, 
drinking, smoking, applying cosmetics and mouth pipetting shall be prohibited in 
the work area. 
"Appendix K-II-E. The facility's emergency response plan shall include provisions 
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