Page 7 - Revision of the Guidelines Subcommittee - 10/15/90 
1 2 nih-rac 
CRITERIOW^ GRSP^ BLI-LS BL2-LS BL3-LS 
12. 
Penetration of a system by agitator shaft 
and measuring devices in a manner that 
minimizes/prevents release of aerosols. 
Not required 
Minimize 
release 
Prevent 
release 
Prevent 
release 
13. 
Foam out control to minimize/prevent 
release of aerosols. 
Not required 
Minimize 
release 
Prevent 
release 
Prevent 
release 
U. 
System located within a designated work 
site (restricted access). 
Not required 
Not required 
Not required^ 
Requi red 
15. 
Provide decontamination facilities for 
personnel. 
Not required 
Not required 
Not required 
Requi red 
16. 
Work site should be designed to contain 
large losses from the system. 
Not required^ 
Required 
Requi red 
Requi red 
17. 
Inactivation of waste solutions and waste 
materials, with respect to their biohazard 
potential . 
Not required 
Required 
Requi red 
Requi red 
18. 
Effluent from sinks and showers should be 
collected and inactivated before release. 
Not required 
Not required 
Not required 
Not required 
19. 
Biosafety manual. 
Not required 
Not required 
Required 
Required 
20. 
Post biohazard sign. 
Not required 
Not required 
Required 
Requi red 
21. 
Devices installed to monitor integrity of 
containment during operation. 
Not required 
Not required 
Requi red 
Required 
22. 
Test system with non- recombinant host prior 
to use. 
Not required 
Not required 
Required 
Required 
23. 
Establish an airlock for controlling 
ingress and egress. 
Not required 
Not required 
Not required 
Required 
24. 
Work side should be maintained under 
negative pressure. 
Not required 
Not required 
Not required - 
Requi red 
25. 
Operate system at low pressure to 
maintain integrity of containment. 
Not required 
Not required 
Not required 
Requi red 
26. 
Work site should be sealable to permit 
fumigation. 
Not required 
Not required 
Not required 
Required 
27. 
Personnel should shower before leaving 
work site. 
Not required 
Not required 
Not required 
Not required 
28. 
Supply and exhaust air to the work area 
should be HEPA-f i Itered. 
Not required 
Not required 
Not required 
Not required^ 
[322] 
Recombinant DNA Research, Volume 14 
