Federal l^egiste^ Vol. 5 ?, Np. 178 / T^urs^ay, September 1 ' )90 / Notifies, ; , ; 37849 
.... . 
NIH-RAC ‘ 
CRITERION* 
GILSP^ 
BL1-LS 
BL2-LS 
BL3-LS 
12. 
Penetration of a systen by agitator shaft . 
and laeasuring devices in a aianher that 
Minimi zes/prevents release of aerosols. 
Not required 
: Minimize 
release 
Prevent 
release 
= prevent 
release 
13. 
Foam out control: to ainimize/plrevent 
release of aerosols. 
Not required 
Minimize 
release . . 
Prevent : 
release 
PrevOfit ' 
=re lease 
H. 
System located within a designated «^rk 
site (restricted access). 
Not required 
Not required 
Not required^ 
Required 
15. 
Provide decontamination f^acilities for, 
personnel. 
.Not required 
Not required 
Not required 
Required 
16. 
Work site should be designed to contain 
large losses from the system. 
Not ‘ requi red^ 
Requi red 
Required 
Required 
17. 
Inactivation of waste solutions and jwaste 
materials, with respect to their bi<^azard 
potential. 
Not require 
Required 
Required 
Retired 
18. 
Effluent frofn sinks and showers ^puld be;, 
collected and inactivated before release. 
Not required 
Not required 
Not required 
Not retired ' 
19. 
Biosafety manual. 
Not required 
Not required 
Required 
Required: 
20. 
Post biohazard sign. 
Not required 
Not required 
Requi red 
Required 
21. 
Devices installed to monitor integrity of 
containment during operation. 
Not required 
Not required 
Requi red 
Required 
22. 
Test system with non- reconi>i riant host prior 
to use. 
Not required 
Not required 
Nequired 
Required 
23. 
Establish an airlock for controlling 
ir^gr^s and egress. 
Not required 
Not required 
Not required 
Required 
24. 
Work side ahoutd be maintained under " 
r>egativ0 pressure. 
Not required 
Not required 
Not required 
Required 
25. 
Operate system at tow pressure to 
maintain integrity of contaimwt. 
Not required 
Not required 
Not requi r(^ 
Re^ir^ 
26. 
Work site should be sealable to permit 
funigation. !' 
Not required 
Not required 
Not required 
Requi red 
27. 
Personnel should shower before leaving , 
work site. 
Not required. 
Not required 
Not required 
Not required 
28. 
Supply and exhaust air to the work area , 
should be HEPA- f i 1 tered. 
Not requi red 
Not required 
Not required 
Not reqqired^ , 
BIU.ING CODE 4140-01-C 
[332] 
Recombinant DNA Research, Volume 14 
