Revision of the Guidelines Subcommittee - \2IQn/9Q 
BL3-LS." 
[Remainder of Introduction remains unchanged.] 
"Appendix K-I - Selection of Physical Containment Levels. 
"The selection of the physical containment level required for recombinant DNA 
research or production involving more than 10 liters of culture is based on the 
containment guidelines established in Part III of the Guidelines. For purposes of 
large-scale research or production, four physical containment levels are 
established. The four levels set contaiiunent conditions at those appropriate for 
the degree of hazard to health or the environment posed by the organism, judged 
by experience with similar organisms unmodified by recombinant DNA techniques 
and consistent with good large scale practices. These are referred to as GLSP, 
BLl-LS, BL2-LS, and BL3-LS. The GLSP (Good Large-Scale Practice) level of 
physical containment is recommended for large-scale research or production 
involving viable, non-pathogenic, and non-toxigenic recombinant strains derived 
from host organisms that have an extended history of safe large scale use. 
Likewise, the GLSP level of physical containment is recommended for organisms 
such as those included in Appendix C that have built-in environmental limitations 
that permit optimum growth in the large scale setting but limited survival without 
adverse consequences in the environment. For those organisms that do not qualify 
for GLSP, the BLl-LS (Biosafety Level 1-Large-Scale) level of physical 
containment is recommended for large-scale research or production of viable 
organisms containing recombinant DNA molecules that require BLl containment 
at the laboratory scale. The BL2-LS (Biosafety Level 2-Large Scale) level of 
physical containment is required for large-scale research or production of viable 
organisms containing recombinant DNA molecules that require BI^ containment 
at the laboratory scale. The BL3-LS (Biosafety Level 3-Large Scale) level of 
physical containment is required for large-scale research or production of viable 
organisms containing recombinant DNA molecules that require BL3 containment 
at the laboratory scale. No provisions are made for large-scale research or 
production of viable organisms containing recombinant DNA molecules that 
require BL4 containment at the laboratory scale. If necessary, these requirements 
will be established by NIH on an individual basis. 
"Appendix K-II-GLSP Level. 
"Appendix K-II-A Institutional codes of practice shall be formulated and 
implemented to assure adequate control of health and safety matters. 
"Appendix K-II-B. Written instructions and training of personnel shall be 
provided to assure that cultures of viable organisms containing recombinant DNA 
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