Revision of the Guidelines Subcommittee - 12/07/90 
molecules are handled prudently and that the workplace is kept clean and orderly. 
"Appendix K-II-C. In the interest of good personal hygiene, facilities (e.g., 
handwashing sink, shower, changing room) and protective clothing (e.g., uniforms, 
laboratory coats) shall be provided that are appropriate for the risk of exposure 
to viable organisms containing recombinant DNA molecules. In addition, eating, 
drinking, smoking, applying cosmetics and mouth pipetting shall be prohibited in 
the work area. 
"Appendix K-II-D. Cultures of viable organisms containing recombinant DNA 
molecules shall be handled in facilities intended to safeguard health during work 
with microorganisms that do not require containment. 
"Appendix K-II-E. Discharges containing viable recombinant organisms shall be 
handled in accordance with applicable governmental environmental regulations. 
"Appendix K-II-F. Addition of materials to a system, sample collection, transfer 
of culture fluids within/between systems, and processing of culture fluids shall be 
conducted in a manner that maintains employee exposure to viable organisms 
containing recombinant DNA molecules at a level that does not adversely affect 
the health and safety of employees. 
"Appendix K-II-G. The facility's emergency response plan shall include provisions 
for handling spills. 
"Appendix K-III-A Spills and accidents which result in overt exposures to 
organisms containing recombinant DNA molecules are immediately reported to 
the laboratory director. Medical evaluation, surveillance, and treatment are 
provided as appropriate and written records are maintained. 
"Appendix K-IV-L. Closed systems and other primary containment equipment 
used in handling cultures of viable organisms containing recombinant DNA 
molecules shall be located within a controlled access area. 
"Appendix K-IV-M-8. The controlled area shall have a ventilation system that is 
capable of controlling air movement. The movement of air shall be from areas 
of lower contamination potential to areas of higher contamination potential. If 
the ventilation system provides positive pressure supply air, the system shall 
operate in a manner that prevents the reversal of the direction of air movement 
or shall be equipped with an alarm that would be actuated in the event that 
reversal in the direction of air movement were to occur. The exhaust air from the 
controlled area shall not be recirculated to other areas of the facility. The exhaust 
air from the controlled area may not be discharged to the outdoors without being 
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