Revision of the Guidelines Subcommittee - 12/07/90 
"Appendix K — Comparison of GLSP and BL-LS Practices^. 
CRITERION^ 
GLSP 
BLl-LS 
BL2-LS 
BL3-LS 
1. 
Formulates and implement institutional codes of practice for safety of 
personnel and adequate control of hygiene and safety measures. 
K-n-A 
G-I 
2. 
Provide adequate written instructions and training of personnel to keep 
workplace clean and tidy and to keep exposure to biological, chemical or 
physical agents at a level that does not adversely affect health and safety 
of employees. 
K-n-B 
G-n 11 
3. 
Provide changing and handwashing facilities as well as protective 
clothing, appropriate to the risk, to be worn during work. 
K-n-c 
G-n-A-l-h 
G-n-B-2-f 
G-II-C-2-i 
4. 
Prohibit eating, drinking, smoking, mouth pipetting, and applying 
cosmetics in the workplace. 
K-n-c 
G-n-A-l-d 
G-II-A-l-e 
G-n-B-l-d 
G-II-B-l-e 
G-II-C-l-c 
G-II-C-l-d 
5. 
Internal accident reporting. 
K-II-D 
K-III-A 
G-II-B-2-k 
and 
G-II-B-2-1 
G-II-C-2-q 
and 
G-II-C-2-r 
6. 
Medical surveillance. * 
NR 
NR 
7. 
Viable organisms should be handled in a system that physically separates 
the process from the external environment (closed system or other 
primary containment). 
NR 
K-III-B 
K-IV-A 
K-V-A 
8. 
Culture fluids not removed from a system until organisms inactivated. 
NR 
K-III-C 
K-IV-B 
K-V-B 
9. 
Inactivation of waste solutions and materials with respect to their 
biohazard potential. 
K-II-E 
K-III-C 
K-IV-B 
K-V-B 
10. 
Control of aerosols by engineering or procedural controls to prevent or 
minimize release of organisms during sampling from a system, addition 
of materials to a system, transfer of cultivated cells, and removal of 
material, products, and effluents from a system. 
Minimize 
Procedure 
K-II-F 
Minimize 
Engineer 
K-III-D 
Prevent 
Engineer 
K-IV-C 
Prevent 
Engineer 
K-V-C 
11. 
Treatment of exhaust gasses from a closed system to minimize or 
prevent release of viable organisms. 
NR 
Minimize 
K-III-E 
Prevent 
K-IV-D 
Prevent 
K-V-D 
12. 
Gosed system that has contained viable organisms not to be opened 
until sterilized by a validated procedure. 
NR 
K-III-F 
K-IV-E 
K-V-E 
13. 
Gosed system to be maintained at as a low pressure as possible to 
maintain integrity of containment features. 
NR 
NR 
NR 
K-V-F 
14. 
Rotating seals and other penetrations into closed system designed to 
prevent or minimize leakage. 
NR 
NR 
Prevent 
K-IV-F 
Prevent 
K-V-G 
15. 
Gosed system shall incorporate monitoring or sensing devices to 
monitor the integrity of containment. 
NR 
NR 
K-IV-G 
K-V-H 
16. 
Validated integrity testing of closed containment system. 
NR 
NR 
K-rv-H 
K-V-I 
17. 
Gosed system to be permanently identified for record keeping purposes. 
NR 
NR 
K-rv-i 
K-V-J 
18. 
Universal biohazard sign to be posted on each closed system. 
NR 
NR 
K-IV-J 
K-V-K 
19. 
Emergency plans required for handling large losses of cultures. 
K-II-G 
K-III-G 
K-IV-K 
K-V-L 
NR = not required 
[ 428 ] 
Recombinant DNA Research, Volume 14 
