Revision of the Guidelines Subcommittee - \2IOni9Q 
"Appendix K - Comparison of GLSP and BI^LS Practices^ (continued). 
CRITERION^ 
GLSP 
BLl-LS 
BL2-LS 
BL3-LS 
20. 
Qosed system to be located in a controlled access area. 
NR 
NR 
K-IV-L 
K-V-M 
21. 
Double doored entry into controlled access area. 
NR 
NR 
NR 
K-V-M-1 
22. 
Surfaces of walls, ceiling, and floors of controlled access area amenable 
to cleaning and decontamination. 
NR 
NR 
NR 
K-V-M-2 
23. 
Penetrations into controlled area sealed to permit decontamination. 
NR 
NR 
NR 
K-V-M-3 
24. 
All utilities and process piping and wiring into controlled area protected 
against contamination. 
NR 
NR 
NR 
K-V-M-4 
25. 
Handwashing facilities within controlled access area in major woric areas 
and near each primary exit. 
NR 
NR 
NR 
K-V-M-5 
26. 
A shower facility to be provided in controlled access area. 
NR 
NR 
NR 
K-V-M-6 
27. 
Controlled area designed to preclude release of culture fluid in the event 
of closed system failure. 
NR 
NR 
NR 
K-V-M-7 
28. 
Controlled access area to have appropriate ventilation system. 
NR 
NR 
NR 
K-V-M-8 
29. 
Personnel entry into controlled access area through specified entry. 
NR 
NR 
NR 
K-V-N-1 
30. 
Personnel change clothing upon entry to and exit from controlled access 
area. 
NR 
NR 
NR 
K-V-N-2 
31. 
Restricted entry of safety trained personnel into controlled access area. 
NR 
NR 
NR 
K-V-N-3 
32. 
No person under 18 permitted in controlled access area. 
NR 
NR 
NR 
K-V-N-4 
33. 
Universal biohazard sign posted on entry and internal doors of 
controlled access area when work and decontamination in progress. 
NR 
NR 
NR 
K-V-N-5 
34. 
Controlled access area to be kept clean and neat. 
NR 
NR 
NR 
K-V-N-6 
35. 
Eating, drinking, smoking, and storage of food prohibited in controlled 
access area. 
NR 
NR 
NR 
K-V-N-7 
36. 
No plants or animals in controlled access area. 
NR 
NR 
NR 
K-V-N-8 
37. 
Insect control program for controlled access area. 
NR 
NR 
NR 
K-V-N-9 
38. 
Access doors to controlled access area to be closed when worir in 
progress. 
NR 
NR 
NR 
K-V-N-10 
39. 
Persons to wash hands when leaving controlled access area. 
NR 
NR 
NR 
K-V-N-11 
40. 
Persons working in controlled access area to be trained in emergency 
procedures. 
NR 
NR 
NR 
K-V-N-12 
41. 
Equipment and materials for management of accidents to be kept in 
controlled access area. 
NR 
NR 
NR 
K-V-N-13 
42. 
Controlled access area to be decontaminated by established procedures 
after accidental release of organisms. 
NR 
NR 
NR 
K-V-N-14 
NR = not required 
Recombinant DNA Research, Volume 14 
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