Revision of the Guidelines Subcommittee - 12/07/90 
"Appendix K — Footnotes. 
"1. This table is derived from the text in Appendices G and K and is not to be used in lieu 
of Appendices G and K. 
"2. The criteria in this grid address only the biological hazard associated with organisms 
containing recombinant DNA Other hazards accompanying the large scale cultivation 
of such organisms (e.g., toxic properties of products; physical, mechanical and chemical 
aspects of downstream processing) are not addressed and must be considered 
separately, albeit in conjunction with this grid. 
"Appendix K - Definitions to Accompany Containment Grid and Proposed 
Modification of Appendix K. 
"Accidental release--The unintentional discharge of a microbiological agent (i.e., 
microorganism or virus) or eukaryotic cell due to a failure in the containment system. 
"Biological barrier- An impediment (naturally occurring or introduced) to the infectivity 
and/or survival of a microbiological agent or eukaryotic cell once it has been released 
into the environment. 
"Closed system-A system, which by its design and proper operation, prevents release 
of a microbiological agent or eukaryotic cell contained therein. 
"Containment-The confinement of a microbiological agent or eukaryotic cell that is 
being cultured, stored, manipulated, transported or destroyed in order to prevent or 
limit its contact with people and/or the environment. Methods used to achieve this 
include: physical and biological barriers and inactivation using physical or chemical 
means. 
”de minimis release-A release of viable microbiological agents or eukaryotic cells that 
does not result in the establishment of disease in healthy people, plants or animals or 
in uncontrolled proliferation of any microbiological agents or eukaryotic cells . 
"Disinfection-A process by which viable microbiological agents or eukaryotic cells are 
reduced to a level unlikely to produce disease in healthy people, plants or animals. 
"Good Large Scale Practice (GLSP) Organism-For an organism to qualify for GLSP 
consideration, it must meet the following criteria : [Reference: Organization for 
Economic Cooperation and Development, Recombinant DNA Safety Considerations y 
1987, p. 34-35. 
"a. The host organism should be non-pathogenic, should not contain adventitious 
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