53260 
Federal Register / Vol. 55, No. 249 / Thursday December 27, 1990 '/ 'Notices 
governmental environmental 
regulations. 
“Appendix K-II-F. Addition of 
materials to a system, sample collection, 
transfer of culture fluids within/between 
systems, and processing of culture fluids 
shall be conducted in a manner that 
maintains employee exposure to viable 
organisms containing recombinant DNA 
molecules at a level that does not 
adversely affect the health and safety of 
employees. 
“Appendix K-II-G, The facility’s 
emergency response plan shall include 
provisions for handling spills. 
“Appendix K-UI-A. Spills and 
accidents which result in overt 
exposures to organisms containing 
recombinant DNA molecules are 
immediately reported to the laboratory 
director. Medical evaluation. 
surveillance, and treatment are provided 
as appropriate and written records are 
maintained. 
“Appendix K-IV-L. Closed systems 
and other primary containment 
equipment used in handling cultures of 
viable organisms containing 
recombinant DNA molecules shall be 
located within a controlled access area. 
“Appendix K-IV-M-0. The controlled 
area shall have a ventilation system that 
is capable of controlling air movement. 
The movement of air shall be from areas 
of lower contamination potential to 
areas of higher contamination potential. 
If the ventilation system provides 
positive pressure supply air, the system 
shall operate in a manner that prevents 
the reversal of the direction of air 
movement or shall be equipped with an 
alarm that would be actuated in the 
event that reversal in the direction of air 
movement were to occur. The exhaust 
air from the controlled area shall not be 
recirculated to other areas of the 
facility. The exhaust air from the 
controlled area may not be discharged 
to the outdoors without being HEPA 
filtered, subjected to thermal oxidation, 
or otherwise treated to prevent the 
release of viable organisms." 
[Remainder of appendix K remains 
unchanged with the exception of the 
following: renumber appendix K-II-A 
becomes K-HI-B; K-II-B becomes K- 
K-II-C becomes K-III-D; K-II-D 
becomes K-III-E; becomes K- 
III-F; K-II-F becomes K-III-G; 
renumber appendix K-UI to appendix 
K-IV; renumber appendix K-IV to 
appendix K-V.] 
BILUNG CODE 4140-01-M 
[438] 
Recombinant DNA Research, Volume 14 
