. Federal Register / Vol. 55, No. 249 / Thursday, December 27, 1990 / Notices 
53261 
13 
"Appendix K - Ckjmpaiison of GLSP and BL-LS Practices’. 
CRITERION^ 
GLSP 
BL1-LS 
BL2-LS 
BL3-LS 
1 
Formulates and implement institutional codes of practice for 
safety of personnel and adequate co.ntrol of hygiene and 
safety measures. 
K-ll-A 
G-l 
2. 
Provide adequate written instructions and training of personnel 
to keep workplace clean and tidy and to keep exposure to 
Oiologica-, chemical or physical agents at a level that does not 
adversely affect health and safety of employees. 
K4I-B 
G-ll 1 1 
3. 
Pro-, de changing and handwashing facilities as well as 
protective clothing, appropriate to the risk, to be worn during 
work. 
K-ii-C 
G4l-A-1-h 
G-ll-B-2-f 
G-il-C-2-i 
4. 
Prohibit eating, drinking, smoking, mouth pipetting, and 
applying cosmetics in the workplace. 
K-il-C 
G-ll-A-1-d 
G-ll-A-1-e 
G-ll-B-1-d 
G-ll-B-1-e 
G-II-C-1-C 
G-ll-C-l-d 
5. 
Internal accident reporting. 
K-ll-D 
K-III-A 
G-ll-B-2-k 
and 
G-ll-B-2-1 
G-ll-C-2-q 
and 
G-ll-C-2-r ' 
6. 
Medical surveillance. 
NR 
NR 
7. 
Viable organisms should be handled in a system that 
physically separates the process from the external 
environment (closed system or other primary containment). 
NR 
K-lll-B 
K4V-A 
K-V-A 
8. 
Culture fluids not removed from a system until organisms 
Inactivated. 
NR 
K-lll-C 
K-IV-B 
K-V-B 
9. 
Inactivation of waste solutions and materials with respect to 
their biohazard potential. 
^ K4I-E 
K-lil-C 
K4V-B 
K-V-B 
10. 
Control of aerosols by engineering or procedural controls to 
prevent or minimize release of organisms during sampling 
from a system, addition of materials to a system, transfer of 
cultivated cells, and removal of material, products, and 
effluents from a system. 
Minimize 
Procedure 
K-ll-F 
Minimize 
Engineer 
K-lll-D 
Prevent 
Engineer 
K4V^ 
Prevent 
Engineer 
K-V-C 
11. 
Treatment of exhaust gasses from a closed system to 
minimize or prevent release of viable organisms. 
NR 
Minimize 
K-lil-E 
Prevent 
K-IV-D 
Prevent 
K-V-D 
12 
Closed system that has contained viable organisms not to be 
opened until sterilized by a validated procedure. 
NR 
K-lll-F 
K-IV-E 
K-V-E 
13, 
Closed system to be maintained at as a low pressure as 
possible to maicstain integrity of ccntainmertt features. 
NR 
NR 
NR 
K-V-F 
14. 
Rotating seals and other penetrations into closed system 
designed to prevent or minimize leakage. 
NR 
NR 
Prevent 
K4V-F 
Prevent 
K-V-G 
15. 
Closed system shall Incorporate monitoring or sensing 
devices to monitor the integrity of containmetU 
NR 
NR 
K-IV-G 
K-V-H 
16. 
Validated integrity testing of closed containment system. 
NR 
NR 
K-IV-H 
K-V-l 
17. 
Closed system to be permanently identified for record keeping 
purposes. 
NR 
NR 
K-IV-I 
K-V-J 
18. 
Universal biohazard sign to be posted on each closed 
system. 
NR 
NR 
K-iy-J 
K-V-K 
IS. 
Emergency plans required for handling large losses of 
cultures. 
K-ll-G 
K-m-G 
K-IV-K 
k-v-l 
NR = not required 
Recombinant DNA Research, Volume 14 
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