g. Baseline pulmonary function tests (arterial blood gases if 
indicated) 
h. Coagulation screen 
i. Cytoxic antibody screen 
j. Surveillance cultures 
k. HIV, CMV, hepatitis, A, B and C screens 
4. Bone marrow aspirate and biopsy 
5. Lumbar puncture for culture and cytology 
6. Bone marrow for karyotype analysis 
7. Skin test: multi-test unit (by IDS) 
8. 5 cc reference serum 
9. If available, bone marrow cells and peripheral blood leukocytes will be 
stored for future studies. 
13.2 During and Post-Transplantation (Months 0-3) 
1. A daily CBC, differential and platelet estimate until self-sustaining and 
then as indicated. 
2. Daily electrolytes during cytoxan and at least weekly SCOT, SGPT, 
total bilirubin, alkaline phosphatase for the first 45 days. 
3. Pulmonary function test (FVC) and cutaneous oximeter readings 
weekly x 3 weeks starting day -9. 
4. EKG daily days -5, -4, -3, -2, before each dose of cytoxan, and again on 
days 1 and 7 post-marrow reinfusion. 
5. Viral, bacterial and fungal cultures as clinically indicated. 
6. Chest x-ray weekly for 3 weeks and as indicated. 
7. Bone marrow aspirate and biopsy day -4-21 and -1-32 post marrow rein- 
fusion for morphology and as indicated, neo-gene content on colony 
culture (see protocol). Lumbar puncture at the end of the third month. 
Recombinant DNA Research, Volume 14 
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