primers shown above. Lanes 1-6 contained 10,000 pg, 1,000 pg, 100 
pg, 10 pg, blank and markers. 10 pg is the amount of DNA in 1.5 
cell equivalents. 0.5 \il of the first round of amplification was 
used in a second round of amplification in which a primer internal 
( 3 ' ) to the 5 ' primer shown above plus the 3 ' primer shown above 
were used for a "heminested amplification” 
1 23 4 5 6 
1 23 4 5 
Figure 1 
Figure 2 
We then used this system to test colonies from CML marrow 
grown in G418 selection after LNL6 exposure. As shown in Figure 2, 
lanes 1-5 contain marker, 1 ng LNL6 DNA, 1 ng HL60 DNA, and DNA 
from 2 colonies, respectively. 
As shown in Figure 2, the product from two of. the colonies 
tested is positive for the NEO template DNA and the negative 
control (HL60) in lane 3 does not contain the NEO product. These 
colonies will be further discussed below in section 2bl. 
b2. Describe anv other material to be used in the preparation 
of the viral supernatant to be administered to the patient ; 
No other materials will be used, except those described above. 
[748] 
Recombinant DNA Research, Volume 14 
