A dedicated laboratory for Bone Marrow Cryopreservation and 
harvesting exists at the M.D. Anderson which is responsible for 
over 250 bone marrow transplants/year. Dr. Christopher Reading, 
whose laboratory is dedicated to the fractionation of human marrow 
for transplantation, is also involved. The Molecular Hematology 
laboratories of Dr. Deisseroth at the U.T. M.D. Anderson Cancer 
Center are approved for BN2 level work. Dr. Deisseroth and his 
colleagues. Dr. Claxton and Dr. Reading, have used PCR to detect 
single copy genes at the level of 10 leukemia cells. Therefore, 
they have the capability of carrying out the PCR experiments for 
NEO detection in G418-resistant colonies. 
C. SELECTION OF PATIENTS : 
Please see Eligibility Requirements of clinical protocol 
(Section 3.0) in Appendix A. 
D. INFORMED CONSENT ; 
Please see informed consent of the clinical protocol (Section 
11.0) in Appendix A. 
E. PRIVACY AND CONFIDENTIALITY : 
Procedures will be followed similar to those outlined on pp. 
83-85 of the PCDD, and developed for the N2-TIL Human Gene Transfer 
Clinical Protocol by Rosenberg and Anderson. Although communication 
with the press will be followed, no information will be devulged 
which will permit identification of the patients or lead to their 
stigmatization. 
II . SPECIAL ISSUES 
A. WHAT STEPS WILL BE TAKEN CONSISTENT WITH POINT I-E ABOVE, 
TO ENSURE THAT ACCURATE AND APPROPRIATE INFORMATION IS MADE 
AVAILABLE TO THE PUBLIC WITH RESPECT TO SUCH PUBLIC CONCERNS THAT 
MAY ARISE FROM THE PROPOSED STUDY? 
The Public Affairs Office of the U.T. M.D. Anderson Cancer 
Center will handle all interactions with the public. 
B. DO YOU OR YOUR FUNDING SOURCES INTEND TO PROTECT UNDER 
PATENT OR TRADE SECRET LAWS EITHER THE PRODUCTS OR THE PROCEDURES 
DEVELOPED IN THE PROPOSED STUDY? 
None of the procedures or materials described in this document 
are subject to restrictions. 
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Recombinant DNA Research, Volume 14 
