3* Distribution of Certified Host-Vectors. 
III.' 
This section should also include new certified HV1 host-vector 
systems. 
Experimental Guidelines. 
B. Containment Guidelines for Permissible Experiments. 
1. Classification of Experiments using the E. coli K-12 Host- 
Vector System. 
a. Shotgun Experiments. 
2. Prokaryotic DNA Recombinants. 
This section remains unnecessarily restrictive and is 
inconsistent with the section on lower eukaryotic DNA's. We 
suggest eliminating the requirement for "extensive cha ra ct e r i ca t i on" 
which could inadvertently eliminate all prokaryotic DNA's other than 
those of E. coli , and T 1 * from the minimum containment of P2 + HV1. 
Surely ".shotgun" experiments to clone DNA from one non-pathogenic 
bacterium to another cannot pose a greater potential risk than 
"shotgun" cloning of plant or fungal DNA into a bacterium. We 
propose that cloning of DNA from non-pathogenic prokaryotes requires 
only P2+HV1 or P1+HV2 containment. Cloning of DNA from Class 2 
organisms could retain the P3+HV2 containment requirement. However, 
care must be taken not to inadvertently include harmless micro- 
organisms as Class 2 agents (see comments below on classification 
of A ctinomycetes ) , since misclassif ication would impose the 
unfortunate and unnecessary requirement of increasing both the 
[Appendix A — 42] 
