permit long term survival. We. propose that in instances where the Mill 
guidelines require safer host systems that spore-defective mutants be 
employed. A strain equivalent to the EK-2 of _E. coli K12 could be 
readily constructed that would have little or no survival outside of 
special laboratory environments. 
We strongly urge that P-1 conditions be permitted for using &. 
subtil is to clone genes fr-om prokaryotes that normally exchange 
genetic information. We as dedicated investigators in this field do 
not believe that this technology in Bacillus s ub till s is hazardous 
to hutuans, animals, or plants. 
Participants in the 7th International 
Spores Conference 
Madison, Wisconsin 
October 5-8, 1977 
[Appendix A — 25] 
