UNIVERSITY OF CALIFORNIA, SAN FRANCISCO 
BERKELEY • DAVIS • IRVINE • LOS AN CELLS • RIVERSIDE • SAN DIEGO • SAN FRANCISCO 
OFFIC E OK ENVIRONMENTAL HEALTH AND SAFETY 
SAN FRANCISCO. CALIFORNIA 9*122 
October 11 , 1977 
Dr. William J. Gartland, Jr. 
Executive Secretary 
Recombinant DNA Molecule Program 
National Institutes of Health 
Bethesda, Maryland 20014 
Dear Dr. Gartland: 
In response to your request for comments on the proposed revised "Guidelines 
for Research Involving Recombinant DNA Molecules", I would like to comment as 
follows: 
1. Autoclaves - It appears there is a relaxation of this require- 
ment for P-3 facilities. I believe that in the current guide- 
lines an autoclave was required within the controlled area - 
now it appears to be required only within the building (Ref.: 
page 11-17(6)). 
Since there are more stringent requirements as you move from 
P-1 to P-4 facilities, i.e., P-1 - no autoclave required, P-2 - 
autoclave within the building, to P-4 - double ended autoclave 
with interlocks, it would seem logical that an autoclave be 
available within the controlled area of a P-3 facility. 
2. Page 11-19 (P-4 level - "Eating, drinking...") - I believe the 
asterisk should be omitted, since this same requirement applies 
to P-1 to P-3 also. 
3. I note the number of appendices have been reduced. I found the 
former appendix "D" especially valuable in providing detailed 
procedures. 
In general, I have found the proposed revision a great improvement over the 
current Guidelines. Congratulations. 
Thank you for the opportunity to review and comment. 
Sine 
:erebs 
rank A. Gohr, Dr. P.H. 
Biosafety Officer 
FAG/ew 
[Appendix A — 6] 
