P roposed Indu s i ons in Revised Guidelines to Cover Recombinant 
D NA Experimentation with Synthetic DNA Segments . Paul Berg 
1) To replace paragraph 1 Page 1-1 (Technical Bulletin on 
Recombinant DNA) . 
I . Introduction- 
The purpose of these Guidelines is to establish procedures 
for constructing and handling organisms and viruses containing 
recombinant DNA molecules. Recombinant DNA molecules are defined 
as molecules which have been constructed outside of living cells 
by joining natural or synthetic DNA segments to DNA molecules that 
can replicate or be integrated into the genome of a living cell. 
However, the recommendations contained herein pertain only to 
organisms and viruses that contain "novel" recombinant DNAs where 
the word "novel" applies to molecules containing a) natural DNA 
segments or reverse trans ipts from species not known to 
exchange DNA by normal physiological processes or b) synthetic 
DNA segments whose nucleotide sequence can or might be expressed 
as a polynucleotide or polypeptide that is not known to be made 
in the chosen host cells. The Director of the NIH, with the 
advice of the Recombinant DNA Molecule Advisory Committee, shall 
prepare a list of those combinations of DNAs (viral, extrachromo- 
somal or chromosomal) which are not considered novel for this 
purpose and are, therefore, not covered by these Guidelines. 
In general recombinant DNA molecules formed from any combination 
of DNAs will not be considered novel when all the components 
are derived from genomes known to replicate within the organism 
used to propagate the recombinant DNA. ^ 
[Appendix A — 84] 
