Chilton - p.5 
in nature. It seems to me that the procedures recommended by 
the Guidelines allow a reasonable possibility of escape of 
novel recombinant DNA. Until data are available which assess 
the probability of such escape, precautions should be taken to 
inactivate transforming activity before discarding samples of 
novel recombinant DNA. Treatment with bleach or acid or dry 
heat should be required for decontamination of glassware. Waste 
materials such as gels and plastic implements should be incinerated. 
Autoclaving is not satisfactory, for DNA can reassociate to form 
native molecules (active in transformation) during the slow 
cooling process. 
[Appendix A — 154] 
