d) In all cases, animals will be tested for the development 
of polyoma HI antibody and tumor formation. 
e) Samples of and feces from mice infected by the oral route 
will be examined for the presence of polyoma virus. 
3. We also contemplate further studies to evaluate the I!, coll 
containing recombinant DNA and will focus on such parameters as growth 
properties, the colonization of germ-free mice, and the transmission and 
mobilization of recombinant plasmid elements. 
Since we do not plan to examine the transcription and translation of 
polyoma DNA in either of the prokaryotic systems under study, we intend to 
make inactivated bacterial lysates available to interested investigators. 
Such studies could conceivably yield important new information about other 
possible mechanisms of biohazard. 
[Appendix C — 86] 
