10 
despite having been designed specifically against such 
survival; (3) such Chi 1776 would have to have been converted 
into a pathogen by the addition of DNA from an organism not 
known to have ever adversely affected man. 
(6) Less than P-4 laboratory protection 
is to be provided for lysates. Affidavit 
119. 
A lysate results from the deliberate treatment of a 
bacterial culture with chemicals which kill all of the host 
cells and release the intracellular DNA, RNA, and protein. 
It is obvious that such materials are inert and non-inf ectidus 
and therefore do not require P-4 containment. Furthermore, 
plasmids cannot transfect coli except under very special 
laboratory conditions which include body temperature, a 
rapidly growing host cell culture, and high calcium concentration. 
For all of these reasons it is unreasonable to view lysates 
as dangerous. 
From previous work with polyoma DNA and other kinds of 
DNA we know that inhalation and ingestion by animals will 
not produce infection. We also know that laboratory workers 
have handled DNA from many kinds of infectious viruses for 
many years without evidence of human infection. 
(7) The Ft. Detrick experiment is a 
prohibited experiment under present NIH 
Guidelines. Memorandum 1|7. 
This statement is simply wrong and no support for this 
erroneous contention is given in the plaintiff's "expert" affidavits. 
Polyoma is not classified as a moderate risk oncogenic virus 
by the National Cancer Institute, but is classified instead 
as a low risk oncogenic virus. Only moderate risk agents 
are excluded by the NIH Guidelines. Indeed, the Guidelines 
recommend use of polyoma virus as a safe procedure for 
cloning other molecules. 
[Appendix C — 152] 
