12 
Provis iona 1 NIH Guidelines: 
"Conjugation minus mutations that 
reduce the ability of conjugative 
plasmids to enter the plasmid-host 
complex . . . should reduce mobili- 
zation of the cloned DNA to 
other strains ..." (Guidelines, p.19) 
*1776 and yl87G . 
The mentioned effects are not alv/ays 
obtained. With sore of the plasmids 
tested, mobilization seems to be 
almost unimpaired v.hen compared to the 
conjugation-plus parent (Curtiss et al . , 
Appendix A, see this paper, §§15, 16, 
17, 18, and 19). 
i 
[Appendix C — 200] 
