22 
§18. Conj ugational ability curing DAP-lcss and/or thyiineless death: 
Transfer of Rl drd l9 by '\1776 to >1763 occurs during starvation 
at a rate proportional to the dcr;or cell death rate (Curtiss et al. , 
p.25). Donor ability is not impaired by starvation conditions to an 
extent greater than viability. 
§19. Conjugational ability cs a function of starvation time in 
non growth liquid media: 
Donor ability is exhibited at 37°C after starvation of 
•f 
Rldrdl9 -containing Xl??6 for 2-1 hours in BSG (Curtiss et al. , p.26). 
§20. Potential for transduc t ional transmission under non-permissive 
conditions: 
a) Both X1776 and can support growth of bacteriophage 
T6 under nonpermissive conditions (Curtiss ot aJh , Figures 29 and 30). 
h) 70-776 is sensitive tt 434, a specialized transducing phage, 
and partially sensitive to PI, a generalized transducing p'.age. It is 
probably sensitive to many uncharcicterized and unknown specialized and 
generalized transducing phages. It is sensitive to BF23, which can 
recombine with T5, which in turn shares part of its host range with 
generalized transducer Tl, the t.rcree phages are morphologically similar, etc.) 
§21. Potential for transf oi~r,c.rion under nonpermissive conditions: 
Although yQ-776 DNA is crspidly degraded by an int ,-stinal extract 
of rats, it is not clear whether :r not this situation is duplicated 
111 anc3 the experiment preso.r.ced does not test this hypothesis (Curtiss 
et al . , p. 27) . 
[Appendix C — 210] 
