IMUA-ia 
-9- 
D". Procedures to be Followed 
1. Records of incoming radioactive materials and laboratory surveys 
for radioactive materials will be maintained in the clean area of 
the Mobile Containment Laboratory (see Page 11 and Section 1(4) of 
the Safety and Operations Manual) in order to permit examination 
at any time by Radiation Safety Branch personnel. 
2. Only working quantities of radioactive materials will be introduced 
in the Class III cabinet system. 
% 
3. Operations with ^^P-orthophosphate will be conducted behind lucite 
shields. 
4. Film badges will be placed in heat-sealed polyethylene bags prior 
to their introdiction into the facility and will be taped to the 
gowns of laboratory personnel. Prior to their removal from the 
facility the baas will be surface decontaminated in the peracetic 
acid pass box and then submitted for routine examination. 
5. Swabs to detect radioactive contamination in the Class III cabinet 
will be placed in a leakproof container which will be surface 
decontaminated. The container and its contents will then be auto- 
claved prior t.o removal from the facility. This procedure will 
then allow for the addition of a scintillation cocktail to the 
counting vial in a P2 laboratory. 
6. Solid and liquid wastes will be separated, decontaminated as 
described in Section 1.2. ( C ) . 1 , Page 18, of the Safety and Operations 
Manual, and disposed of by the Radiation Safety Branch in the usual 
fas hi on. 
IX. TRANSFER OF MATERIALS OUT OF CLASS III CABINETS 
A. Potentially Hazardous Material 
1. Bacterial cultures containing P-lambda or P-plasmid recombinant DNAs 
that have been propagated in the Mobile Containment Laboratory will 
be transferred to Building 550, Frederick Cancer Research Center 
(FCRC) for inoculation into mice and hamsters. Packaging and trans- 
portation of all recombinant DNA materials will be carried out in 
accordance with the relevant sections of the NIH Guidelines. 
2. 'Such material will be placed in a primary container which will be 
surface decontaminated. The primary container will then be sealed 
in a leakproof secondary container in the Class III cabinet. 
3. The leakproof container will be transferred out of the cabinet via 
the dunk tank and surface decontami natea with peracetic acid spray 
during transit through the pass box. 
4. The container will be labeled with the warning: "Open Only Under 
P4 Containment." 
[Appendix C — 239] 
