b. Chemistry survey including electrolytes, liver 
function tests, calcium, magnesium, creatinine, 
BUN, CPK, pancreatic enzymes. 
c. CBC , differential count, PT, PTT, platelet 
count. 
d. Urine analysis and culture. 
e. Hepatitis screen. 
f. HIV titer. 
g. Chest x-ray. 
h. Electrocardiogram. 
i. 45 ml of clotted blood for serum storage and 45 
ml of anti-coagulated blood for mononuclear cell 
cryopreservation . 
9.2 Treatment 
Patients will be analyzed with routine blood and chemistry 
analysis. A chest x-ray will be initially subsequently if 
needed. During the gene transfer protocol, patients will be 
monitored closely in the Clinical Research Center. Vital signs 
will be measured every 15 minutes prior to, during, and after the 
infusion for at least two hours or until the patient is stable. 
If the systolic blood pressure drops below 80 mm/Hg or the oxygen 
saturation drops below 90%, the infusion will be terminated 
immediately. 
9.3 Post-treatment 
Patient's status will be evaluated by physical examination, 
laboratory studies, and appropriate imaging scans prior to 
treatment, and monthly after the first month or as needed to 
Recombinant DNA Research, Volume 18 
[23] 
