Protective Effects of Different Enhancers in 
Combination with Rev M10 in T Leukemia Cells 
E 
CL 
U 
o 
< 
I- 
cr 
CONTROL 
RSV PROMOTER 
HIV LTR 
RSV TAR 
DAYS POST INFECTION 
Figure 2. Control CEM cells and OEM cells stably expressing 
RevMlO from various enhancers (RSV, HIV LTR or RSV-tar) , were 
infected with HIV Bru (1:500 ratio TCID 50 HIV: CEM cells), for 2 
hrs. Cells were then washed and resuspended in fresh media. 
Culture supernatants were then assayed for RT activity at various 
times post infection. Cultures were split every 3 to 5 days 
dependent on growth rate. The plasmid proposed for use in the 
biolistics protocol is RSV-tar, whose structure and sequence is 
described in Appendix V. 
[34] 
Recombinant DNA Research, Volume 18 
