/cB activation and the function of the HIV tat -I gene. In two 
cell lines, EL-4 and Jurkat, the expression of Rev M10 did not 
alter the ability of these cells to produce IL-2 following 
mitogen stimulation (16) . In addition, no changes have been 
detected in any of these cell lines with respect to cell 
proliferation or cell surface antigen expression, including CD4 
and CD3 (data not shown) . We have also examined the effects of 
constitutive Rev M10 expression in primary T lymphocytes of 
murine or human origin. Genetically modified human peripheral 
blood T cells resistant to HIV infection were assayed for 
immunologic function. Rev M10 and ARev M10 transduced-selected 
peripheral blood T cells were assayed for tumor necrosis factor a 
secretion (TNFa) following stimulation with immobilized anti-CD3. 
No differences in the level or kinetics of TNF cytokine secretion 
were observed between the two populations (Fig. 4, panel a). The 
cytolytic activity of these same populations was assayed by anti- 
CD3 directed killing of P815 tumor targets. In these 
experiments, both Rev M10 and ARev M10 populations of T cells 
exhibited similar levels of cytotoxicity (Fig. 4, panel b) . Rev 
M10 transduced cells also showed the ability to secrete 
interferon 7 (IFN-*) (data not shown) . 
[36] 
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A) anti-CD3 stimulated TNF release 
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B) anti-CD3 redirected cytotoxicity 
■ ARevMIO 
Recombinant DNA^Research, Volume 18 
F-T Ratio 
