VEOGE NETICS 
TAB 
SUMMARY OF ALVAC APPENDICES 
A 
Report demonstrating the host-range restricted nature of ALVAC-RG 
(vCP65) in human cell lines in culture. 
B 
Comparison of the abilities of ALVAC (CPpp) and ALVAC-RG (vCP65) to 
adapt to growth in two mammalian cell lines (Virogenetics report #7-06- 
RAB). 
C 
Comparison of yield of ALVAC (CPpp) grown in avian and non-avian cells 
at 31 oC, 37o C, and 39oC, Showing that ALVAC (CPpp) is unable to 
replicate in non-avian cells regardless of the temperature of incubation. 
D ; 
Report illustrating the host-range restricted nature of ALVAC-RG (vCP65) 
by comparing viral replication in mice and canaries. 
E 
Recovery of virus from inoculation site— two parts: 1) a safety study in 
which squirrel monkeys were inoculated with ALVAC (CPpp) and 
ALVAC-RG (vCP65) via four different routes and sites were swabbed for 
recovery of inoculated virus. 
2) a study of mice in which analysis of viral recovery from site of 
inoculation was performed by titration of QT35 cells and by amplification 
of viral sequences by PCR analysis. 
F 
Comparison of the virulence of canarypox virus (Rentschler strain) and of 
ALVAC-RG (vCP65) in canary birds. 
G 
"NYVAC: A Highly Attenuated Strain of Vaccinia Virus", Tartaglia et al., 
Virology , 188:217-232 (1992). This paper presents safety data on ALVAC 
(CPpp) and ALVAC-RG (vCP65) and compares them to various strains of 
vaccinia and vaccinia-based constructs. 
H 
"Highly attenuated Poxvirus Vectors in AIDS Vaccine Development", Cox 
et al. Submitted for publication. This paper presents data showing the 
ability of ALVAC-based constructs to express extrinsic immunogens and 
elicit protective immune responses. 
I 
"Efficacy studies on a canarypox-rabies recombinant virus", Taylor et al.. 
Vaccine, 9:190-193 (1991). This paper presents data of an efficacy study 
done on dogs, cats and mice. 
J 
"Protection of Cats Against Feline Leukemia Virus by Vaccination with a 
Canarypox Virus Recombinant, ALVAC-FL", Tartaglia et al., manuscript 
submitted to J. Virology. 
Recombinant DNA Research, Volume 18 
[167] 
