Recombinant DNA Advisory Committee - 09/9-10/93 
molecule genes in E. coli K-12 (see Appendix F). 
"..Appendix C-II. Experiments Involving E. coli K-12 Host-Vector Systems... 
"...Exceptions. The following categories are not exempt from the NIH Guidelines: 
(i) experiments described in Section III -A which require specific RAC review and 
NIH and IBC approval before initiation, (ii) experiments described in Section IH- 
B which require NIH (ORDA) and IBC approval before initiation, (iii) 
experiments involving DNA from Class 3, 4, or 5 organisms [1] or cells known to 
be infected with these agents may be conducted under containment conditions 
specified in Section III-C-2 with prior IBC review and approval, (iv) large-scale 
experiments (e.g., more than 10 liters of culture), and (v) experiments involving 
the cloning of toxin molecule genes in E. coli K-12 (see Appendix F). 
"...Appendix C-III. Experiments Involving Saccharomyces Host-Vector Systems... 
"...Exceptions. The following categories are not exempt from the NIH Guidelines: 
(i) experiments described in Section III-A which require specific RAC review and 
NIH and IBC approval before initiation, (ii) experiments described in Section III- 
B which require NIH (ORDA) and IBC approval before initiation, (iii) 
experiments involving DNA from Class 3, 4, or 5 organisms [1] or cells known to 
be infected with these agents may be conducted under containment conditions 
specified in Section III-C-2 with prior IBC review and approval, large-scale 
experiments (e.g., more than 10 liters of culture), and experiments involving the 
cloning of toxin molecule genes in E. coli K-12 (see Appendix F). 
"...Appendix C-IV. Experiments Involving Bacillus subtilis Host-Vector Systems... 
"...Exceptions. The following categories are not exempt from the NIH Guidelines: 
(i) experiments described in Section III-A which require specific RAC review and 
NIH and IBC approval before initiation, (ii) experiments described in Section III- 
B which require NIH (ORDA) and IBC approval before initiation, (iii) 
experiments involving DNA from Class 3, 4, or 5 organisms [1] or cells known to 
be infected with these agents may be conducted under containment conditions 
specified in Section III-C-2 with prior IBC review and approval, large-scale 
experiments (e.g., more than 10 liters of culture), and experiments involving the 
cloning of toxin molecule genes in E. coli K-12 (see Appendix F). 
"...Appendix C-V. Extrachromosomal Elements of Gram Positive Organisms... 
"...Exceptions. The following categories are not exempt from the NIH Guidelines: 
(i) experiments described in Section III-A which require specific RAC review and 
NIH and IBC approval before initiation, (ii) experiments described in Section III- 
B which require NIH (ORDA) and IBC approval before initiation, (iii) large-scale 
experiments (e.g., more than 10 liters of culture), and (iv) experiments involving 
the cloning of toxin molecule genes in E. coli K-12 (see Appendix F.)" 
Recombinant DNA Research, Volume 18 
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