Schema 
Stable or PD 
Other Therapies 
4 -5 Cycles Induction Chemotherapy 
\ 
-PR or CR 
Peripheral Blood Stem Cell Harvest 
Bone Marrow harvest 
clxlO 8 nucleated cells/Kg 
from bone marrow 
consider for transplant on 
another Medicine Branch study or 
consider other therapies 
>lxl0 8 nucleated cells/Kg 
from both bone marrow 
and peripheral blood 
30% used to 
separate CD 34+ 
fraction 
CD34+ fractions transfected 
with the MDR-1 gene 
70% 
cryopreserved 
ICE chemotherapy 
administered 
Reinfuse transduced and 
non-transduced BM and PB stem cells 
Sample peripheral blood and bone 
marrow at multiple time points during 
and after engraftment to test for 
presence of MDR-1 gene 
A. 
Begin daily G-CSF 
■ Restage Breast Ca 1 
month post-ABMT 
I 
Residual or Progressive 
Disease 
Follow for presence 
of MDR-1 gene in BM and WBC and 
for breast Ca recurrence 
Relapse 
Treatment with taxol or vinblastine 
Biopsy relapsed tumor to 
test tumor for MDR-1 gene 
[276] 
Recombinant DNA Research, Volume 18 
