The intracerebral (i.c.) injections were given to neonatal mice, whereas the intranasal (i.n.) and 
intraperitoneal (i.p.) injections were given to 40-day-old mice. 
b SE, standard error. 
c Mice were challenged at 14 days p.i. with 10 4 PFU given intraperitoneally (i.p.) of the virulent 
SFV L10 strain. 
‘‘nd, not done; na, not applicable. 
'Lesions were scored at 14 days post-injection. Two of three mice infected with activated or 
unactivated SQL virus showed focal areas of gliosis and lymphoid perivascular cuffing in the 
grey and white matter areas of the brain. Shrunken neurons with fragmented nuclei and 
markedly eosinophilic cytoplasm were occasionally observed in the lesions. 
f SQL, virus derived from SFV-SQL cDNA; lacZ/Hl, SFV3-lacZ recombinant virus packaged 
with Helper 1; lacZ/H2, SFV3-lacZ recombinant virus packaged with Helper 2; SFV4, virus 
derived from SFV cDNA clone. L10, virulent prototype strain of SFV. Amounts of activated 
or unactivated virus used are indicated in parentheses. 
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Recombinant DNA Research, Volume 18 
