C. METHODS TO INACTIVATE SFV 
Studies on the inactivation of SFV were performed to determine which reagents will 
effectively inactivate the virus, given a BL2 laboratory setting. The reagents evaluated 
were BACDOWN detergent disinfectant, BACDOWN antimicrobial handsoap, chlorine 
bleach, NP-40, Triton X-100, and sodium dodecyl sulfate (SDS) solutions. UV light 
inactivation was also studied. 
Procedures'. 
Viral stock solution of SFV3 LacZ/Helper 2 were prepared by electroporation in 
BHK-21 as described in section IHA. 
Activation and infection were performed using standard procedures described in 
section III A. 
Reagents : 
Reagent Stock Solutions 
BACDOWN™ 
BACDOWN™ Handsoap 
Chlorine bleach 
NP-40 
Triton X-100 
SDS 
15ml/L 
lOml/L 
lOOml/L 
1% Solution 
1% Solution 
1% Solution 
Controls: 
Plating efficiency of activated SFV-3 LacZ/Helper 2 virus was determined for 
each of the reagents above to determine the concentration that did not interfere 
with viral infection of cells. The following concentrations of reagents had no 
effect on virus infectivity: 
Reagent Reagent Cone. 
BACDOWN Detergent 0.45ml/L 
BACDOWN Handsoap 0. lml/L 
Chlorine bleach 0 . 1 7ml/L 
NP-40 .033% 
Triton X-100 .033% 
SDS .033% 
Recombinant DNA Research, Volume 18 
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