NP-40 
Inactivation Cone. 
Final conc.on cells No. of 6-eal Dositive cells/6wells 
0.5% NP-40 
.0017% 
0 
0.17% NP-40 
.00055% 
0 
0.05% NP-40 
.00017% 
6 
PBS control 
2X10 5 
PBS control 
.0017% 
2X10 5 
Conclusion: 0.17% 
NP-40, or higher, 
completely inactivates 2x10 s infectious 
units of SFV. 
TRITON X-100 
Inactivation Cone. Final cone. on cells No. of <3-gal positive cells/6wells 
0.5% Triton X-100 
.0017% 
7 
0.17% Triton X-100 
.00055% 
300 
0.05% Triton X-100 
.00017% 
1000 
PBS control 
-2X10 5 
PBS control 
.0017% 
-2X10 5 
Conclusion: 0.5% Triton X-100 reduces SFV infectious units by —30,000 fold. 
SDS 
Inactivation Cone. Final cone, on cells No. of fl-gal positive cells/6wells 
0.5% SDS .0017% 0 
0.17% SDS .00055% 0 
0.05% SDS .00017% 100 
PBS control 2X10 5 
PBS control .0017% 2X10 5 
Conclusion: 0.17% SDS, or higher, completely inactivates 2x10 s infectious units 
of SFV. 
Recombinant DNA Research, Volume 18 
[479] 
