BACDOWN ANTIMICROBIAL HANDSOAP 
Inactivation Cone. Final conc.on cells No. of fl-gal positive cells/6wells 
5 ml/L 
.017ml/L 
0 
1.7 ml/L 
.0055ml/L 
0 
0.5 ml/L 
.0017ml/L 
0 
PBS control 
2X10 5 
PBS control 
.017ml/L 
1X10 3 
Conclusion: 0.5 ml/L, or higher, of BACDOWN Handsoap completely inactivates 
2x10 s infectious units of SFV. 
CHLORINE (HOUSEHOLD) BLEACH 
Inactivation Cone. Final conc.on cells No. of 6-gal positive cells/6wells 
50 ml/L .017ml/L 0 
17 ml/L .0055ml/L 0 
5 ml/L .0017ml/L 7 
PBS control 2X1 0 s 
PBS control .017ml/L 1X10 3 
Conclusion: 17 ml/L, or higher, of chlorine bleach completely inactivates 2x10 s 
infectious units of SFV. 
UV LIGHT 
The killing of SFV by UV light was evaluated using 100/zl aliquots (lxlO 6 
infectious units) of viral stock (~2xl0 7 infectious units/ml) dispensed into 35 mm 
dishes. With lids removed, virus aliquots were exposed to a UV light source 
(30W Germicidal light, Sylvania), for 0, 5, 10, 20, 30, 40, 50 or 60 minutes. 
Treated virus samples (100/il) were then removed and plated on BHK 21 cells, 
following the standard protocol for infection (Section mA). Cells were incubated 
18 hrs at 37 °C then stained for /3-gal expression as described in section IHA). 
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Recombinant DNA Research, Volume 18 
