Results: 
Time of UV exposure ('mini 
No. of g-gal pos. cells 
0 
5 
10 
20 
30 
40 
50 
60 
>5xl0 5 
1 
0 
0 
0 
0 
0 
0 
Conclusion: UV light exposure for 10 minutes or longer completely inactivates 
5x10 s infectious units of SFV. 
In summary, SFV on surfaces can be completely inactivated by a 10-minute, or longer, 
exposure to UV light; SFV in solution is inactivated by incubating for 5 minutes at room 
temperature with any of the following reagents at, or above, the indicate concentrations. 
Reagent 
Minimum Effective 
Concentration 
BACDOWN Detergent 
NP40 
7.5 ml/L 
0.17% 
SDS 
0.17% 
Chlorine bleach (5%) 
BACDOWN Handsoap 
0.5 ml/L 
17 ml/L 
Recombinant DNA Research, Volume 18 
[481] 
