Test Schedule 
Tests and Procedures 
History and exam, tumor 
meas., ht, wt, PS 
Vital signs (pulse, blood 
pressure, temperature) 
Hematology group 
WBC, HgB, PLT 
Chemistry group (SGOT, alk. 
phos., dir. and t. bili, creat.) 
PT, Ptt 
Electrocardiogram 
Chest x-ray 
CT scan & ultrasound 
Tumor biopsy-guided by 
hepatic ultrasound 
Urinalysis 
HbAg, HIV titer 
HLA-B7 phenotype 
Assay for cytoxic T-cells and 
antibody 2 
Peripheral blood sample 
Antinuclear antibodies 
Serum preg. test 6 
<15 days prior During course of Rx Follow-up: at 8 wks post Rx 
to registration days 3, 15, and 29 7 and q 8 wks until PROG, then 
8-12 wks 
XX X 
X 1 
XX X 
XX X 
X Days 15 and 29 only Day 57 7 
X 
X 
X Days 15 and 29 only X 
Days 15 and 29 only Days 43 5 ' 7 and 57 5 ' 7 
X 
X 
X 
X X 
X 3 X 4 Day 5 7 7 
X Day 29 only Day 57 7 
X 
1. Prior to each biopsy and treatment and them every 15 minutes until stable. 
2. Biopsy tissue will be assayed for the presence of the HLA-B7 gene by PCR. Expression of the 
HLA-B7 gene will be looked for immunohistochemically. When sufficient materials are present, 
attempts to assess the development of cytolytic T-cell response to the cells of the tumor will be 
evaluated. Tissue will be "snap" frozen in liquid nitrogen (LN) and sent in LN to Dr. P.CX. 
Roche, Immunhistochemical Core Lab, Hilton Building. 
3. 45ml of clotted blood for serum storage and 45ml of anti coagulated blood for mononuclear cell 
cry op reservation will be obtained (sent at room temperature to Dr. H.A. Homburger, Cellular 
Immunology core Laboratory, Hilton Building). 
4. 20ml of clotted blood will be obtained to look for development of antibodies, and 20ml of anti 
coagulated blood for mononuclear cell cryopreservation will be obtained (sent at room 
temperature to Dr. H.A. Homburger, Cellular Immunology Core Laboratory, Hilton Building). 
5. Only for patients receiving 2 or 3 injections of DNA on schedule B. 
6. For women of childbearing potential only. Must be done within 7 days prior to registration. 
7. Days 29, 43, and 57 are approximate. The goal will be to obtain the studies as close as possible 
to 4, 6, and 8 weeks +/- 2 days. 
Recombinant DNA Research, Volume 18 
[771] 
