PROTOCOL: Adoptive Immunotherapy of Melanoma with Activated Lymph 
Node Cells Primed in Vivo with Autologous Tumor Cells Transduced with the 
IL-4 Gene 
I 
1.0 Pr QtPPQ l Schema Protocol 
SCHEMA : Patients with advanced melanoma will undergo surgical retrieval of tumor for 
use as a tumor vaccine. A tumor cell line will be established in culture and subsequently 
transduced with a retroviral vector containing the IL-4 gene. The transduced line will be 
screened for IL-4 production and subsequently irradiated with 5,000 cGy. Patients will be 
vaccinated with 10 7 -10 8 irradiated transduced tumor cells intradermally in 1 or 2 sites. 
Surgical removal of draining lymph nodes (LN) 7 to 10 days after vaccination will be 
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Recombinant DNA Research, Volume 18 
