Attachment IV - Page 2 
C. Pathological, ecological, and physiological characteristics. 
D. Methods for detection and monitoring natural populations of this 
microorganism. Describe the sensitivity of these methods. 
III. Characteristics of the modified microorganism to be released into the 
environment. 
A. Source of the DMA sequences to be inserted into or used to modify 
the microorganism to be released into the environment. 
1. Identification, taxonomy, source, and strain of organism donating 
the DNA. 
2. General biological characteristics including relevant genetics. 
3. Pathological, ecological, and physiological characteristics. 
B. Genetic manipulations: describe the method(s) by which the DNA vector 
has been constructed and introduced. Include diagrams as appropriate. 
1. Describe the nature of the DN\ inserted or modified by the genetic 
manipulations. Document the amount of any donor DNA in excess of 
the amount required for the minimal effective genetic alteration in 
the final construction. Predict whether any changes in pathological, 
ecological, physiological, or genetic properties of host organism 
would be anticipated as the result of the excess donor DMA. 
2. Describe the amount of any vector DNA remaining in the final 
construction. Predict whether any changes in pathological, 
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