Attachment IV - Page 3 
ecological, physiological, or genetic properties of the host 
organism would be anticipated as the result of any remaining 
vector DNA. 
3. Describe the method of introduction of the DNA and selection of 
altered organisms. If microorganisms are used to introduce 
vectors or cure vectors themselves: 
a. Indicate how they compare with wild-type strains; 
b. If disarmed pathogens are used to transmit the vector, indicate 
factors that will most likely prevent these microorganism from 
regaining or acquiring pathogenic potential; 
c. If the vector is likely to survive independently of the host(s), 
refer to this possibility and provide any available data to 
assess the probability of transfer to other organisms; 
d. If a microorganism will only be used to introduce a vector (s) 
or are vectors themselves, document the means and sensitivity 
of the techniques by which the presence or the absence of the 
microorganism will be assessed in the final construction to be 
released . 
C. Properties of the modified organism. 
1. Describe anticipated properties of the final population of engineered 
organisms with emphasis on the desired effect and any other possible 
pathological , ecological, physiological , or genetic effects. 
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