Attachment IV - Page 4 
2. Provide data related to any anticipated or non-ant icipated effects 
of the modified microorganism from microcosm, greenhouse, and/or 
growth chamber experiments that simulate natural conditions. The 
methods of detection and sensitivity of sampling techniques and 
periodicity of sampling should be indicated. These studies should 
include assessment of the following items. 
a. Survival of the microorganism to be released. 
b. Replication of the microorganism to be released. 
c. Dissemination routes of the microorganism. 
3. Document the means and sensitivity of the techniques by vhich the 
presence or the absence of donor organism will be assessed in the 
final population of microorganisms to be released into the environ- 
ment. 
IV. Release into the environment. 
A. Describe the experiment involving release of the modified organism into 
the environment. Provide information, including diagrams, of the 
experimental location and the surrounding environs . Information 
provided should include pertinent plant, soil, water, aerobiologic, 
and/or other data to assess the containment or spread of the 
microorganism. 
B. Indicate containment procedures in the event of accidental release as 
well as intentional release and procedures for emergency termination of 
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