Attachment V - Page 2 
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b. Ecological niches of the organisms; 
c. Generations times in natural ecosystems; 
d. Involvement in significant environmental processes (e.g., mineral 
cycling, cellulose and lignin degradation, nitrogen fixation); 
e. Effects on population levels of other organisms in the ecosystem; 
f. Nature and degree of any antibiotic resistance; 
g. Other potentially significant physiological or ecological traits; 
h. Stability of these traits." 
Dr. Pirone noted that it was probably impossible to test for the data required 
in itan 3-e. 
Dr. Tolin suggested that items one through eight in Dr. Vidaver's document are 
generic; item number nine is oriented towards plants. Dr. Tolin suggested 
item one through eight might cover general information requests; separate 
sections might then be tailored to the specific areas such as plant associated 
microorganisms, mining organisms, and pollution control organisms. 
Noting that she had based her proposed document on the format followed in the 
document Points to Consider Under Appendix LT , Dr. Vidaver asked if a similar 
appendix would be developed for microorganisms. Dr. Milewski contrasted the 
development of Points to Consider Under Appendix L which was written as an 
adjunct to Appendix L to this document for which there is no appendix. She 
suggested the working group might first attempt to write such an appendix for 
microorganisms, but thought an appendix could be developed out of a "Points 
to Consider" document. She suggested the working group develop a "Points to 
Consider" document and then develop an appendix if they see that as desirable. 
Dr. Lacy agreed with the approach of first developing a "Points to Consider" 
document; he felt an appendix written to cover most microorganisms would be 
preferable to developing one appendix for each projected application of modified 
microorganisms, and a single appendix cculd best be developed frcm "Points to 
Consider. " 
Dr. Milewski suggested the working group review Dr. Vidaver's document item by 
item. Dr. Milewski suggested the first sentence of the first paragraph of 
Dr. Vidaver's document be deleted. Dr. Pirone suggested the title be changed 
to "Points to Consider for Submission of Informaticn on Release of Microorganisms 
Modified by Recombinant DMA Techniques into the Environment." 
Dr. Milewski, in response to a question fran Dr. Lacy, suggested the second 
sentence of the first paragraph should read: 
"Experiments in this category cannot be initiated without submission of 
relevant information on the proposed experiments to NIH for review toy the 
appropriate working group, RAC and specific approval by NIH." 
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